ASTM E3527 - 26

1.1 There is an ongoing need to maintain, preserve, and standardize microbiological cultures for research. This guide is focused on three parts of microbe preservation:

1.2 In this guide, methods for characterization, storage, maintenance, and stabilization of a broad taxonomy of microorganisms are reviewed, including vegetative bacteria, bacterial spores, mycobacteria, fungi, and viruses.

1.3 This guide is intended to assist in the development of standardized methods for preservation of microorganisms by indicating actions to be considered at each stage of accession and preservation.

1.4 This guide does not purport to detail the specific workflow necessary to developing a standardized method for preservation of a specific microorganism. Literature review and empirical evidence may be considered in the process of developing a final method specific to the microorganism of interest.

1.5 Units—The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.