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CEN/TR 15215-2:2006

Characterization of sludges - Detection and enumeration of Salmonella spp. in sludges, soils, soil improvers, growing media and biowastes - Part 2: Liquid enrichment method in selenite-cystine medium followed by Rapport-Vassiliadis for semi-quantitative Most Probable Number (MPN) determination

Edition date: 2006-01-25
In Force
Available languages: German, English, French
Summary: This part of the CEN Technical Report method describes a method to detect and semi-quantitatively determine Salmonellae in sludges, soils, soil improvers, growing media and biowastes in accordance with the requirements of the European Sewage Sludge Regulation Revision of Directive 86/278/EEC (3rd Draft, CEN/TC 308 - doc525). The fully defined scope will be determined after the proposed validation trials have been agreed and carried out. The method has a limit of detection of approximately 1cfu/g wet weight sample. NOTE The objective is to cover untreated and treated sludges, soils, soil improvers, growing media, biowastes and associated materials.

Dieses Dokument legt in Übereinstimmung mit den Anforderungen der europäischen Bestimmung über Klärschlamm, Überarbeitung der Richtlinie 86/278/EWG (3. Entwurf, CEN/TC 308 – doc 525) ein Verfahren zum Nachweis und zur halbquantitativen Bestimmung von Salmonellen in Schlämmen, Böden, Bodenverbesserungsmitteln, Kultursubstraten und Bioabfällen fest. Der vollständig definierte Anwendungsbereich wird bestimmt, nachdem die vorgeschlagenen Validierungsuntersuchungen vereinbart und durchgeführt sind. Das Verfahren hat eine Nachweisgrenze von etwa 1 KBE/g Feuchtprobenmasse. ANMERKUNG Der vorgesehene Anwendungsbereich umfasst die Untersuchung von unbehandelten und behandelten Schlämmen, Böden, Bodenverbesserern, Kultursubstraten und Bioabfall umfasst.

La présente partie du Rapport Technique CEN décrit une méthode permettant la détection des Salmonella et leur détermination semi-quantitative dans les boues, sols, amendements du sol, supports de culture et biodéchets, conformément aux exigences contenues dans la révision de la Directive 86/278/CEE (3ème version, CEN/TC 308 – doc. 525), portant sur la Réglementation européenne relative aux boues d’épuration. Le domaine d’application exact ne sera établi qu’une fois les essais de validation proposés pris en compte et réalisés. La méthode a une limite de détection d’environ 1 ufc/g de masse d’échantillon de boue humide. NOTE L’objectif est de couvrir le domaine des boues traitées et non traitées, sols, amendements du sol, supports de culture, biodéchets et produits assimilés.
ICS: 07.100.99-Other standards related to microbiology
CTN: CEN/TC 308 - 6289

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