DIN EN 15851:2010-07
Foodstuffs - Determination of aflatoxin B<(Index)1> in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection; German version EN 15851:2010
| Fecha edición: |
2010-07-01
En Vigor
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| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | This European Standard specifies a method for the determination of aflatoxin B<(Index)1> in baby food by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 0,07 µg/kg to 0,18 µg/kg. A test portion is extracted with a mixture of methanol and water. The extract is filtered, diluted with phosphate buffered saline (PBS) to a specified solvent concentration, and applied to an immunoaffinity column containing antibodies specific to aflatoxin B1. Aflatoxin B1 is purified and concentrated on the column and removed from the antibodies using methanol as eluent. Aflatoxin B1 is quantified by reverse-phase high performance liquid chromatography (RP-HPLC) with post column derivatization (PCD) involving bromination followed by fluorescence detection. The post column derivatization is achieved with either electrochemically generated bromine or with pyridinium hydrobromide perbromide (PBPB). |
| Keywords: | Aflatoxin|Analysis|Baby foods|Calibration fluids|Cereal products|Cereals|Chemical analysis and testing|Chemicals|Children foods|Diet|Food inspection|Food products|Foodstuff|High performance liquid chromatography|HPLC|Immunoaffinity column|Liquid chromatography|Test equipment|Test report|Testing |
| ICS: | 67.060 - Cereales, leguminosas y productos derivados, 67.230 - Alimentos preenvasados y cocinados |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN 15851:2010 |
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Reemplazo Normas |
Reemplaza a DIN EN 15851:2008-09 |
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