DIN EN 16939:2017-10
Animal feeding stuffs - Methods of sampling and analysis - Detection of tylosin, spiramycin and virginiamycin - Thin Layer Chromatography and bioautography; German version EN 16939:2017
| Fecha edición: |
2017-10-01
En Vigor
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| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | The method makes it possible to detect and identify spiramycin, tylosin and virginiamycin in animal feeding stuffs (feed raw materials of mainly plant origin and compound feeds) excluding mineral feeds and premixtures. The limit of detection is about 2 mg/kg for spiramycin, 1 mg/kg for tylosin and 1 mg/kg for virginiamycin. In some milk replacers, it can be slightly higher than 1 mg/kg for virginiamycin. Reported limits of detection are probably little overestimated but were fully validated during the collaborative study. In each laboratory, each day of analysis, spiked blank samples at 1 mg/kg for spiramycin and virginiamycin and at 0,5 mg/kg for tylosin are analysed for checking lower detection limits. These lower limits of detection are achievable, but should be established with an in-house validation first. That method should be used as a qualitative screening and/or a post-screening method (after microbiological plate test, for example). The follow-up of the antibiotics presence may be done by other analytical technics (LC and/or LC-MS technics). For confirmatory purposes, LCMS is required. |
| Keywords: | Animal feed|Antibiotics|Chromatography|Definitions|Detection limits|Investigation methods|Investigations|Mixed animal fodder|Sampling methods|Screening-method|Thin-layer chromatography|Tylosin|Verification|Virginiamycin |
| ICS: | 65.120 - Alimentos para animales |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN 16939:2017 |
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Reemplazo Normas |
Reemplaza a DIN EN 16939:2015-12 |
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