DIN EN 17683:2023-04
Animal feeding stuffs - Methods of sampling and analysis - Determination of pyrrolizidine alkaloids in animal feeding stuff by LC-MS/MS; German version EN 17683:2023
| Fecha edición: |
2023-04-01
En Vigor
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| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | This document describes a method for the quantitative determination of pyrrolizidine alkaloids (PA) in complete and supplementary feed and in forages by liquid chromatography tandem mass spectrometry (LC-MS/MS) after solid phase extraction (SPE) clean-up. The method has been successfully validated in a collaborative trial for the matrices complete feed for horses, supplementary feed for horses, supplementary feed for rodents, hay, alfalfa and grass silage. Validation was carried out for the PA. It was demonstrated that the PA isomeric pairs senecivernine and senecionine as well as senecivernine-N-oxide and senecionine-N-oxide cannot be determined individually due to insufficient chromatographic separation. However, the sums of the individual PA of the isomeric pairs were quantified with sufficient reproducibility. Co-elution of other PA-isomers not included in the scope of the method shall be taken into account. |
| Keywords: | Agricultural products|Alkaloids|Analysis|Animal feed|Determination of content|Extraction|Extraction methods of analysis|Investigation methods|Liquid chromatography|Mass spectrometry|Sampling methods|Solid phase extraction|Toxin |
| ICS: | 65.120 - Alimentos para animales |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN 17683:2023 |
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Reemplazo Normas |
Reemplaza a DIN EN 17683:2021-07 |
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