DIN EN ISO 12966-1:2015-10

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014); German version EN ISO 12966-1:2014 + AC:2015

Fecha edición:	2015-10-01 En Vigor
Idiomas disponibles:	Inglés, Alemán
Resumen:	This part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, free and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEs). The qualitative and quantitative determination of the composition of fatty acids by gas liquid chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of fats and oils, or fatty foodstuffs after the extraction of the oil from the matrix. The bound fatty acids of the triacylglycerols (TAGs) and, depending on the esterification method, the free fatty acids (FFA) and other lipids, are converted to fatty acid methyl esters (FAMEs), which are determined by capillary gas chromatography. Depending on the number of different fatty acids (theoretically more than 50 different fatty acids can be present) capillary columns with a length of 10 m to 100 m are used for a separation. The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols, to fatty acid esters, free fatty acids, soaps and other fatty compounds. With this suite of standards, FAMEs from C4 to C26 can be determined, including saturated fatty acid methyl esters, cis- and transmonounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. For the determination of short chain fatty acids, isopropyl and butyl esters are often used so as to avoid interferences with the solvent peak and in order to reduce differences in detector responses.
Keywords:	Agricultural products\|Agriculture\|Animal fats\|Animal oils\|Applications\|Composition\|Conglomerates\|Esterification\|FAME\|Fats\|Fatty acid methyl esters\|Fatty acid methylesters\|Fatty acids\|Food products\|Gas chromatography\|Investigations\|Methods\|Methyl esters\|Oils\|Production\|Testing\|Vegetable fats\|Vegetable oils
ICS:	67.200.10 - Aceites y grasas de origen animal y vegetal
CTN:
Equivalencia Internacional	Idéntica EN ISO 12966-1:2014/AC:2015 Idéntica ISO 12966-1:2014
Reemplazo Normas	Reemplaza a DIN EN ISO 12966-1:2015-03 Reemplaza a DIN EN ISO 15304:2002-07 Reemplaza a DIN EN ISO 15304 Berichtigung 1:2006-09

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