DIN EN ISO 15216-2:2019-12
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 2: Method for detection (ISO 15216-2:2019); German version EN ISO 15216-2:2019
| Fecha edición: |
2019-12-01
En Vigor
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|---|---|
| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | This document specifies a method for detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. |
| Keywords: | Animal feed|Bacterial viruses|Contamination|Definitions|Food inspection|Food products|Food testing|Hepatitis|Investigations|Laboratory testing|Laboratory tests|Microbial foods|Microbiological analysis|Microorganisms|Noroviruses|PCR|Polymerase|Polymerase chain reaction|Qualitative analysis|Real-time|Surfaces|Verification|Viruses |
| ICS: | 07.100.30 - Microbiología de los alimentos |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN ISO 15216-2:2019 Idéntica ISO 15216-2:2019 |
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Reemplazo Normas |
Reemplaza a DIN EN ISO 15216-2:2018-06 Reemplaza a DIN CEN ISO/TS 15216-2:2014-09;DIN SPEC 10051-2:2014-09 |
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