DIN EN ISO 17601:2024-09
Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO/DIS 17601:2024); German and English version prEN ISO 17601:2024 / Note: Date of issue 2024-08-09
| Fecha edición: |
2026-06-01
En Vigor
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| Idiomas disponibles: | Alemán, Inglés |
| Resumen: | ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups. It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms. |
| Keywords: | Analysis|Composition of the ground|Definitions|DNA|Extraction methods of analysis|Genes|Measurement|Measuring techniques|Microbiology|Microorganisms|PCR|Polymerase chain reaction|Quantitative analysis|Sampling methods|Soil analysis|Soil sampling|Soil surveys|Soils |
| ICS: | 13.080.30 - Propiedades biológicas de los suelos |
| CTN: | |
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Reemplazo Normas |
Es reemplazada por DIN EN ISO 17601:2026-06 |
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