DIN EN ISO 19040-2:2023-12
Water quality - Determination of the estrogenic potential of water and waste water - Part 2: Yeast estrogen screen (A-YES, Arxula adeninivorans) (ISO 19040-2:2018); German version EN ISO 19040-2:2022
| Fecha edición: |
2023-12-01
En Vigor
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| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha. Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %. This method is applicable to: - fresh water; - waste water; - sea water; - brackish water; - aqueous extracts and leachates; - eluates of sediments (fresh water); - pore water; - aqueous solutions of single substances or of chemical mixtures; - drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng/l and 3 ng/l 17 |
| Keywords: | Biochemical methods of analysis|Biological|Biological analysis and testing|Biological tests|Definitions|Degradability|Dilution|Drinking water|Genetically modified organisms|Oestrogen|Organisms|Potable water|Potentials|Research methods|Sampling methods|Sewage|Testing|Water analysis|Water pollution|Water quality|Water samples|Water testing|Yeast |
| ICS: | 13.060.70 - Determinación de las propiedades biológicas del agua |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN ISO 19040-2:2022 Idéntica ISO 19040-2:2018 |
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Reemplazo Normas |
Reemplaza a DIN EN ISO 19040-2:2022-09 |










