DIN EN ISO 23418:2022-09
Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of bacteria - General requirements and guidance (ISO 23418:2022); German version EN ISO 23418:2022
| Fecha edición: |
2022-09-01
En Vigor
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|---|---|
| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | This international standard specifies minimum requirements for generating and analyzing whole-genome sequence (WGS) data obtained from foodborne bacteria. These requirements are applicable to any sequencing platform or chemistry. This process may include the following stages: handling of bacterial cultures, genomic DNA Isolation, sequencing library preparation, sequencing, and assessment of raw DNA sequence read quality and storage, bioinformatic analysis, including methods such as high quality single nucleotide polymorphism (hqSNP) analysis, core genome and whole genome multi-locus genotyping (cgMLST, wgMLST), and bioinformatic pipeline validation; and Metadata capture and sequence repository deposition. |
| Keywords: | Analysis|Bacteria|Data acquisition|Data analysis|Data exchange|Definitions|DNA|Food inspection|Food products|Genetics|Genomic information|Interoperability|Laboratories|Laboratory tests|Metadata|Microbiology|Minimum requirements|Sample preparation|Sequencing|Specification (approval)|Typing procedures|Validation |
| ICS: | 07.100.30 - Microbiología de los alimentos |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN ISO 23418:2022 Idéntica ISO 23418:2022 |
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Reemplazo Normas |
Reemplaza a DIN EN ISO 23418:2020-10 |
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