DIN EN ISO 6579-4:2025-06
Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 4: Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR) (ISO 6579-4:2025); German version EN ISO 6579-4:2025
| Fecha edición: |
2025-06-01
En Vigor
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| Idiomas disponibles: | Inglés, Alemán |
| Resumen: | This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. |
| Keywords: | Agricultural products|Analysis|Bacteria|Chain reactions|Definitions|Determination|Food inspection|Food products|Food testing|Identification|In vitro|Investigation methods|Methods of analysis|Microbiological analysis|Microbiology|Polymerase|Polymerase chain reaction|Production|Salmonella|Sampling methods|Testing|Verification |
| ICS: | 07.100.30 - Microbiología de los alimentos |
| CTN: | |
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Equivalencia Internacional |
Idéntica EN ISO 6579-4:2025 Idéntica ISO 6579-4:2025 |
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Reemplazo Normas |
Reemplaza a DIN EN ISO 6579-4:2024-01 |
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